Targeted next-generation sequencing to diagnose drug-resistant tuberculosis: a systematic review and meta-analysis

This systematic review and meta-analysis aimed to identify targeted next-generation sequencing (NGS) assays for diagnosing drug-resistant tuberculosis. The study screened 2920 reports from 2005 to 2022, focusing on detecting mutations associated with resistance to tuberculosis drugs. The combined sensitivity and specificity of targeted NGS across all drugs were 94.1% and 98.1%, respectively. The study concluded that targeted NGS is highly sensitive and specific for detecting drug resistance, but there is a lack of data on performance for some recommended drugs.