Comparison of methods proposed for monitoring cefotaxime-resistant Escherichia coli in the water environment

The World Health Organization TriCycle Protocol has been used to monitor antimicrobial resistance (AMR) in water environments. The protocol, which uses tryptone bile x-glucuronide medium and incubation at 35°C, has been found to be effective in detecting cefotaxime-resistant E. coli. However, the study found that the TriCycle Protocol can be adjusted to other standard methods and can improve specificity by reducing false-positive identification of cefotaxime-resistant E. coli by adjusting incubation conditions.