Systematic mapping of antibiotic cross-resistance and collateral sensitivity with chemical genetics

A study using chemical genetics data from the Escherichia coli single-gene deletion library in 40 antibiotics found that bacteria can develop resistance to one antibiotic, leading to cross-resistance and collateral sensitivity. The study expanded the number of known interactions by threefold and validated 64/70 inferred interactions using experimental evolution. The study also applied collateral-sensitive drug pairs to reduce antibiotic-resistance development.